Journal: Scientific Reports

Article Title: Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs

doi: 10.1038/s41598-020-61015-0

Figure Lengend Snippet: Lack of E2 envelope glycoprotein on the surface of some SINV-infected cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV nsP3 (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.

Article Snippet: Infected cells were identified with a rabbit polyclonal antibody to SINV nsP3 and CF633-conjugated anti rabbit IgG(H + L) (Biotium).

Techniques: Infection, Staining, Membrane